abstract：:The human ryk tyrosine kinase cDNA was originally identified as a PCR-amplified cDNA fragment (JTK5) from K562 leukemia cells and found to represent a ubiquitously expressed gene (Partanen et al., 1990). The open reading frame of human ryk, reported here, encodes a novel type of putative tyrosine kinase of 607 amino a...

journal_title：Oncogene

authors： Tamagnone L,Partanen J,Armstrong E,Lasota J,Ohgami K,Tazunoki T,LaForgia S,Huebner K,Alitalo K

更新日期：1993-07-01 00:00:00

abstract：:Fos protein heterodimerizes through one surface of an alpha-helical domain called the leucine zipper. We have investigated the effect of destabilizing this domain by multiply substituting small residues of its non-interacting surface with glycine. Ternary complex formation between mutated Fos, Jun and DNA was determin...

journal_title：Oncogene

authors： Nicklin MJ,Zechner D,Kislyakova T

更新日期：1993-07-01 00:00:00

abstract：:wnt genes encode secretory glycoproteins that have been implicated in growth control and development in mice, frogs and insects. In this report we examine properties of two wnt genes recently identified in the nematode Caenorhabditis elegans. The first gene, Ce-wnt-1, was previously identified by a polymerase chain re...

journal_title：Oncogene

authors： Shackleford GM,Shivakumar S,Shiue L,Mason J,Kenyon C,Varmus HE

更新日期：1993-07-01 00:00:00

abstract：:Human xeroderma pigmentosum (XP) fibroblasts were transformed with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The transformed cells, called ASKMN, were immortalized, grew in agar and were tumorigenic in nude mice. A trp-met oncogene was identified in ASKMN cells, after transfection of high molecular weight DNA on 3T...

journal_title：Oncogene

authors： Michelin S,Daya-Grosjean L,Sureau F,Saïd S,Sarasin A,Suárez HG

更新日期：1993-07-01 00:00:00

abstract：:We have developed a transplantation system that allows us to introduce oncogenes into mouse mammary epithelial cells in culture and then to reconstitute an epithelial tree in vivo from the genetically altered cells. Introduction of the neu oncogene, a transforming homologue of the human proto-oncogene c-erbB-2, produc...

journal_title：Oncogene

authors： Bradbury JM,Arno J,Edwards PA

更新日期：1993-06-01 00:00:00

abstract：:We have isolated rat cDNAs that encode two related receptor-like tyrosine kinases. One of these receptors, TIE-1, is the rat homolog of a recently described human receptor-like kinase termed TIE (Partanen et al., 1992). The related TIE-2 receptor has the same organization of amino acid sequence motifs characteristic o...

journal_title：Oncogene

authors： Maisonpierre PC,Goldfarb M,Yancopoulos GD,Gao G

更新日期：1993-06-01 00:00:00

abstract：:Structural rearrangements involving chromosome 13 are frequently seen in B-cell chronic lymphocytic leukaemia. The presence of reciprocal translocations involving 13q14 in 10-15% of cases pinpoints the location of a gene important in leukaemogenesis. In order to characterise the exact location of the 13q14 breakpoint,...

journal_title：Oncogene

authors： Hawthorn LA,Chapman R,Oscier D,Cowell JK

更新日期：1993-06-01 00:00:00

abstract：:The hepatitis B virus (HBV) X protein (pX) stimulates transcription regulated by cis-acting elements that control many viral and cellular genes, including the c-myc and the c-fos proto-oncogenes. Using several c-fos promoter deletion mutants, we found the serum-responsive element (SRE) located at -315, the modified TP...

journal_title：Oncogene

authors： Avantaggiati ML,Natoli G,Balsano C,Chirillo P,Artini M,De Marzio E,Collepardo D,Levrero M

更新日期：1993-06-01 00:00:00

abstract：:This report describes a new polymorphism, in intron 3 of the p53 gene, which consists of a single repeat of 16 nucleotides, absent in the published wild-type p53 gene sequence. In the Caucasian population tested (n = 82), 28% of individuals were heterozygotes for this polymorphism. Using PCR-based analysis, we were ab...

journal_title：Oncogene

authors： Lazar V,Hazard F,Bertin F,Janin N,Bellet D,Bressac B

更新日期：1993-06-01 00:00:00

abstract：:Wild-type (wt) p53 acts as a tumor suppressor, while certain mutant type (mt) p53 may exhibit 'oncogenic' function. We have recently demonstrated that human papillomavirus type 18 (HPV-18) E6 can partially overcome the growth-suppressive effects of wt p53, but it remains unclear what role p53 plays in cervical carcino...

journal_title：Oncogene

authors： Chen TM,Chen CA,Hsieh CY,Chang DY,Chen YH,Defendi V

更新日期：1993-06-01 00:00:00

abstract：:To investigate a possible role for c-Jun homodimers in c-Jun-mediated transformation, we designed two chimeric c-Jun derivatives, called c-Juneb1 and c-Jungcn4. In these chimeric derivatives the natural dimerization domain of c-Jun was replaced by the heterologous homodimerization domain of the Epstein-Barr virus EB1 ...

journal_title：Oncogene

authors： Castellazzi M,Loiseau L,Piu F,Sergeant A

更新日期：1993-05-01 00:00:00

abstract：:rho genes have been found in both lower and higher eucaryotes. They code for proteins of 21 kDa, highly conserved in evolution, which belong to the superfamily of ras GTPases. Among the members of this superfamily there are proteins with a regulatory function, such as ras, and proteins involved in vesicular traffickin...

journal_title：Oncogene

authors： Perona R,Esteve P,Jiménez B,Ballestero RP,Ramón y Cajal S,Lacal JC

更新日期：1993-05-01 00:00:00

abstract：:We determined whether proteins encoded by the nm23/nucleoside diphosphate (NDP) kinase gene, a potential metastasis-suppressor gene, are expressed on the cell surface. Monoclonal antibodies (mAb) specific for nm23-H1 or H2 proteins were prepared using the corresponding fusion proteins with glutathione S-transferase (G...

journal_title：Oncogene

authors： Urano T,Furukawa K,Shiku H

更新日期：1993-05-01 00:00:00

abstract：:The C-terminal src kinase (Csk) is responsible for the phosphorylation of the carboxy-terminal tyrosine of several tyrosine kinases of the Src family. This phosphorylation site has a negative regulatory function. Csk is unique among the members of the protein tyrosine kinase family because it lacks the conserved tyros...

journal_title：Oncogene

authors： Bougeret C,Rothhut B,Jullien P,Fischer S,Benarous R

更新日期：1993-05-01 00:00:00

abstract：:Previous reports have indicated that the cellular slime mold Dictyostelium discoideum possesses two ras genes (rasG and rasD) and one rap gene (rap1). All three genes are developmentally regulated, with each showing a different pattern of transcription during the Dictyostelium life cycle. To establish whether there ar...

journal_title：Oncogene

authors： Daniel J,Spiegelman GB,Weeks G

更新日期：1993-04-01 00:00:00

abstract：:While screening a chicken kidney cDNA library for the normal homolog of the yes oncogene, we isolated a clone that encodes a novel non-receptor type protein tyrosine kinase of the Src family. We named this gene product Yrk (York), as an acronym for Yes-related kinase. As predicted from the cDNA sequence, the Yrk prote...

journal_title：Oncogene

authors： Sudol M,Greulich H,Newman L,Sarkar A,Sukegawa J,Yamamoto T

更新日期：1993-04-01 00:00:00

abstract：:The requirement for cis-acting DNA sequences for transcriptional activity of the human lck type I promoter was investigated in two human cell lines that express type I transcripts, the leukemic T-cell line, Jurkat, and the colon carcinoma line, SW620. Transient transfection assays in Jurkat and SW620 cells revealed ne...

journal_title：Oncogene

authors： Leung S,McCracken S,Ghysdael J,Miyamoto NG

更新日期：1993-04-01 00:00:00

abstract：:We have previously reported on the identification of a cDNA (placenta growth factor, PlGF) coding for a novel angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). Biochemical and functional characterization of PlGF derived from t...

journal_title：Oncogene

authors： Maglione D,Guerriero V,Viglietto G,Ferraro MG,Aprelikova O,Alitalo K,Del Vecchio S,Lei KJ,Chou JY,Persico MG

更新日期：1993-04-01 00:00:00

abstract：:pp60c-src, a cellular tyrosine kinase homologous to the retroviral v-src oncogene, becomes transiently activated during mitosis. Activation is accompanied by phosphorylation of three sites in the amino-terminal regulatory domain of the protein, threonine 34, threonine 46 and serine 72. These sites can be phosphorylate...

journal_title：Oncogene

authors： Kaech S,Schnierle B,Wyss A,Ballmer-Hofer K

更新日期：1993-03-01 00:00:00

abstract：:Several motifs are conserved in the extracellular domain of the cloned chains of the recently described cytokine receptor superfamily. One of them, usually close to the transmembrane region, is the 'WS motif'. Its function remains unknown, but it has been recently shown that the integrity of this motif is essential fo...

journal_title：Oncogene

authors： Bénit L,Charon M,Cocault L,Wendling F,Gisselbrecht S

更新日期：1993-03-01 00:00:00

abstract：:Platelet-derived growth factor (PDGF) and vascular endothelial growth factor define a family of dimeric proteins characterized by eight conserved cysteine residues involved in disulfide bonds. Thirteen non-cysteine residues conserved among the platelet-derived/vascular endothelial growth factors were individually muta...

journal_title：Oncogene

authors： Maher DW,Strawn LM,Donoghue DJ

更新日期：1993-03-01 00:00:00

abstract：:The current model for colorectal tumorigenesis defines four specific mutations (activation of a ras proto-oncogene and inactivation of the APC, p53 and DCC tumor-suppressor genes) that accumulate in a colonic epithelial cell as it progresses towards a carcinoma. However, further mutations must be needed for progressio...

journal_title：Oncogene

authors： Young J,Leggett B,Ward M,Thomas L,Buttenshaw R,Searle J,Chenevix-Trench G

更新日期：1993-03-01 00:00:00

abstract：:The gene for von Recklinghausen neurofibromatosis type 1 (NF1) was recently identified by positional cloning and found to encode a protein with sequence similarity to a family of eucaryotic GTPase-activating proteins (GAPs). Expression of the NF1-GAP-related domain (NF1GRD) has been shown to complement yeast strains d...

journal_title：Oncogene

authors： Gutmann DH,Boguski M,Marchuk D,Wigler M,Collins FS,Ballester R

更新日期：1993-03-01 00:00:00

abstract：:Cancer therapy drugs, such as diamminedichloroplatinum (cisplatin), mitomycin C, etoposide and a number of other compounds, as well as energy-rich radiation, are known to act on cellular DNA. These agents are shown to induce nuclear accumulation of the so-called tumor-suppressor protein p53 in fibroblastoid cells, as ...

journal_title：Oncogene

authors： Fritsche M,Haessler C,Brandner G

更新日期：1993-02-01 00:00:00

abstract：:cDNA clones of the ski-related gene, sno, were isolated from a chicken cDNA library and sequenced. In contrast to the human system, from which two forms of sno cDNAs have been isolated, we obtained only one type of chicken sno cDNA, that encoding snoN. The coding region for chicken snoN was inserted into the retrovira...

journal_title：Oncogene

authors： Boyer PL,Colmenares C,Stavnezer E,Hughes SH

更新日期：1993-02-01 00:00:00

abstract：:Frequent allelic losses of chromosome 3p in lung cancer have been reported in a number of studies, and we previously demonstrated that 3p21.3 is one of the common regions of deletion in lung cancers and renal cell carcinomas. To further define a region containing the putative tumor suppressor gene, we performed Southe...

journal_title：Oncogene

authors： Yamakawa K,Takahashi T,Horio Y,Murata Y,Takahashi E,Hibi K,Yokoyama S,Ueda R,Takahashi T,Nakamura Y

更新日期：1993-02-01 00:00:00

abstract：:Exposure of normal adult human skin to doses of UV irradiation that induced mild sunburn resulted in the rapid appearance of p53 protein in the epidermis and superficial dermal fibroblasts. Immunohistological analysis with a panel of antibodies established that while p53 staining was not seen in normal skin it appeare...

journal_title：Oncogene

authors： Hall PA,McKee PH,Menage HD,Dover R,Lane DP

更新日期：1993-01-01 00:00:00

abstract：:Two alternatively spliced mouse lymphocyte and brain ltk cDNAs predict small transmembrane tyrosine kinases that use CUG translational start codons and that differ upstream of their transmembrane segment. A recently isolated human neuroblastoma ltk cDNA, in contrast, includes a regular AUG start codon and predicts a m...

journal_title：Oncogene

authors： Snijders AJ,Haase VH,Bernards A

更新日期：1993-01-01 00:00:00

abstract：:The oncoprotein c-Myc contains two dimerization motifs- the helix-loop-helix (HLH) and the leucine zipper (LZ) - through which c-Myc specifically dimerizes with Max. We substituted regions of the c-Myc HLH and LZ motifs with the corresponding regions of structurally related proteins that do not interact with Max. Spec...

journal_title：Oncogene

authors： Davis LJ,Halazonetis TD

更新日期：1993-01-01 00:00:00

abstract：:We have previously reported the isolation of several temperature-sensitive (ts) mutants of F9 cells. Further investigations showed that some mutants were induced to differentiate at non-permissive temperature of cell growth, accompanied by changes in the expression of various genes, whereas others were not. During the...

journal_title：Oncogene

authors： Kosaka M,Iwai SA,Nishina Y,Sumi T,Nishimune Y

更新日期：1992-12-01 00:00:00

abstract：:A novel protein tyrosine kinase (PTK) substrate, p120, has been previously implicated in ligand-induced signaling through the epidermal growth factor, platelet-derived growth factor and colony-stimulating factor 1 receptors, and in cell transformation by p60v-src. We have isolated a near full-length cDNA encoding muri...

journal_title：Oncogene

authors： Reynolds AB,Herbert L,Cleveland JL,Berg ST,Gaut JR

更新日期：1992-12-01 00:00:00

abstract：:A two-stage (initiation and promotion) model of chemical transformation of mouse embryonic fibroblasts was used to elucidate the molecular mechanisms of tumor promotion in vitro. C3H10T1/2 cells which had been initiated with a subcarcinogenic dose (0.5 micrograms ml-1) of benzo[a]pyrene (B[a]P) were isolated after 12-...

journal_title：Oncogene

authors： Wu WS,Lin JK,Wu FY

更新日期：1992-11-01 00:00:00

abstract：:Overexpression of members of the myc family of oncogenes contributes to the development of many vertebrate malignancies. Although several functions for myc gene products have been proposed, the targets for Myc activity during oncogenesis or normal development remain to be identified. Oocytes of Xenopus laevis, which a...

journal_title：Oncogene

authors： Gibson AW,Ye R,Johnston RN,Browder LW

更新日期：1992-11-01 00:00:00

abstract：:The myb proto oncogene product (c-Myb) is a transcriptional regulator and its expression and function are tightly linked to the cellular entry into S phase and DNA synthesis. It has been shown [Venturelli, D., Travali, S. & Calabretta, B. (1990). Proc. Natl. Acad. Sci. USA, 87, 5963-5967] that inhibition of T-cell pro...

journal_title：Oncogene

authors： Sudo T,Miyazawa H,Hanaoka F,Ishii S

更新日期：1992-10-01 00:00:00

abstract：:The RCAS retroviral vector was used to express chicken and mouse cellular Jun proteins in chicken embryo fibroblasts. Both mouse and chicken proteins induced foci of transformed cells with low to moderate efficiency compared with viral Jun, but were as effective as the viral protein in promoting anchorage-independent ...

journal_title：Oncogene

authors： Wong WY,Håvarstein LS,Morgan IM,Vogt PK

更新日期：1992-10-01 00:00:00

abstract：:Transcription from the rat transforming growth factor alpha (TGF-alpha) promoter initiates at multiple sites within a 200-bp G+C-rich region that lacks TATA and CAAT motifs but contains multiple potential binding sites for the transcription factor Sp1. In the present study, we used deletion analysis to establish the 5...

journal_title：Oncogene

authors： Chen X,Azizkhan JC,Lee DC

更新日期：1992-09-01 00:00:00

abstract：:A dominant negative mutant of Ras, M17 Ras, was used to study the role of Ras in receptor coupling of Raf-1 and B-Raf protein serine/threonine kinases (PSKs). We found that mutant Ras blocks serum- and 12-O-tetradecanoyl phorbol 13-acetate-induced activation of Raf-1 kinase in NIH3T3 cells and Raf-1 as well as B-Raf P...

journal_title：Oncogene

authors： Troppmair J,Bruder JT,App H,Cai H,Liptak L,Szeberényi J,Cooper GM,Rapp UR

更新日期：1992-09-01 00:00:00

abstract：:To explore the molecular mechanisms by which retinoic acid inhibits oncogenic transformation, we have examined the effects of retinoic acid on the polyomavirus-induced transformation of rat fibroblasts. Treatment of rat F111 fibroblasts with high concentrations of retinoic acid (10(-6) M) partially inhibited the abili...

journal_title：Oncogene

authors： Talmage DA,Lackey RS

更新日期：1992-09-01 00:00:00

abstract：:Alterations in expression of c-myb can have profound effects on the growth and differentiation of hematopoietic cells. Thus, it is important to understand the mechanisms by which c-myb is regulated during hematopoietic cell differentiation. Previous studies pertaining to the regulation of c-myb have been carried out w...

journal_title：Oncogene

authors： Boise LH,Gorse KM,Westin EH

更新日期：1992-09-01 00:00:00

abstract：:A search for protein tyrosine kinases expressed during murine cardiogenesis resulted in the isolation of a novel tyrosine kinase, designated tek, which maps to mouse chromosome 4 between the brown and pmv-23 loci. The deduced amino acid sequence of tek predicts that it encodes a putative receptor tyrosine kinase that ...

journal_title：Oncogene

authors： Dumont DJ,Yamaguchi TP,Conlon RA,Rossant J,Breitman ML

更新日期：1992-08-01 00:00:00